CONSTRUCTION AND EXPRESSION
OF QUARTET RECOMBINANT PEPTIDE SURFACTANT FOR EOR APPLICATION

Authors

  • Cut Nanda Sari PPPTMGB "LEMIGAS"
  • Usman Usman PPPTMGB "LEMIGAS"
  • Refiana Lestary Institut Teknologi Bandung (ITB)
  • Riesa Khairunnisa W.R. Institut Teknologi Bandung (ITB)
  • Leni Herlina PPPTMGB "LEMIGAS"
  • Syafrizal Syafrizal PPPTMGB "LEMIGAS"
  • Tati Kristianti Institut Teknologi Bandung (ITB)
  • Sony Suhandono Institut Teknologi Bandung (ITB)

DOI:

https://doi.org/10.29017/SCOG.39.3.98

Keywords:

peptide surfactant, quartet recombinant, SUPEL, thermal stability, EOR

Abstract

The main drawback of the SUPEL peptide surfactant product which has been developed for EOR application is it isunstable at a high temperature. This research is aimed at generating the prototype of peptide surfactant construction in recombinant by stringing up 4 SUPEL linier sequences. Quartet recombinant technology can produce the peptide surfactant characterized as reversible biosurfactant, which is active at high temperature but inactive at low temperature. Multiple SUPEL Construction (MSC) that was developed in this research is using synthetic DNA and producing SUPEL in 4 sequences that can flip at normal temperature and can open when heated. SDS PAGE analysis results show that MSC construction can be expressed by inducting IPTG and cell harvested at 90°C. This research proves that construction and expression of the SUPEL quartet has been achieved by producing the peptide at an ideal size.

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Published

05-10-2018

Issue

Vol. 39 No. 3 (2016)

Section

Articles

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