Dilute Acid Pretreatment And Enzymatic Hydrolysis Of Lignocellulosic Biomass For Butanol Production As Biofuel

Devitra Saka Rani, Cut Nanda Sari

Abstract


Biobutanol is one of the promising biofuel for substituting gasoline. Biobutanol produced from biomass fermentation using solventogenic clostridia which are able to convert a wide range of carbon sources to fuels such as butanol. Therefore, lignocellosic biomass has great potential as fermentation substrate for biobutanol production. Lignocellosic biomass should be hydrolized before fermentation by a pretreatment process and enzymatic hydrolysis. The various lignocellulosic biomass pretreatment will infl uence in butanol production depending on fermentable sugars content. The objective of this research is to get potential lignocellulosic biomass using dilute acid pretreatment and enzymatic hydrolysis process for biobutanol production. Eight types of biomass from sugarcane bagasse, rice straw, rice husk, empty fruit bunch (EFB) of palm oil, corn cob, pulp waste, traditional market organic waste, and microalgae were used in this experiment. After hydrolysis, the high result of total fermentable sugars in corn cobs, bagasse, rice straw, and rice husk, shows good opportunity of these biomass to be used as fermentation feedstocks for biobutanol production. In addition, pulp waste, organic waste, and microalgae are prospective as raw material but require more appropriate treatment either for to break down the cellulose/hemicellulose or to enhance reducing sugar content. Fine milling and delignifi cation have no signifi cant effect on cellulosic biomass conversion into fermentable sugars. Therefore, the production cost can be reduced. In order to enhance the sugar content and reduce the formation of inhibitor product, it is necessary to examine dilute acid pretreatment variations and appropriate operating conditions of enzymatic hydrolysis process


Keywords


biofuel, biobutanol, lignocellulosic biomass, dilute acid pretreatment, enzymatic hydrolysis

Full Text:

PDF

References


Aleksic, S. 2009. “Butanol Production From

Biomass”, Thesis, Chemical Engineering,

Youngstown State University, US.

Adney, B. and J. Baker. 1996. “Measurement

of Cellulase Activities”. LAP-006 NREL

Analytical Procedure, National Renewable

Energy Laboratory, Colorado.

Anwar, N. A. Widjaya, S. Winardi. 2008.

“Study of Enzymatic Hydrolysis of Rice

for Hydrogen Production Using Mixed

Cellulases”. http://digilib.its.ac.id/public/

ITS-Research-11652-195209161980031002-

Paper3.pdf.

Carvalho, R.N.L., 2009. “Dilute Acid and

Enzymatic Hydrolysis of Sugarcane Bagasse

for Biogas Production”, Dissertation, Instituto

Superior Tecnico, Lisboa.

Ezeji, T.C., N. Qureshi, and H.P. Blaschek.

“Bioproduction of Butanol from Biomass:

from Genes to Bioreactors”, Current Opinion in

Biotechnology, 18:220–227.

Gómez-Sandra, M.R. Andrade Rafael R,

Santander Carlos G, Costa Aline C, Maciel

Filho Rubens, 2010. “Pretreatment of Sugarcane

Bagasse with Phosphoric and Sulfuric Diluted

Acid for Fermentable Sugars Production by

Enzymatic Hydrolysis”, In: E. Bardone and

A. Viglia (eds). 321 Chemical Engineering

Transactions, Vol. 20.

Harmsen, P., W. Huijgen, L. Bermudez,

and R. Bakker, 2010. “Literature Review of

Physical and Chemical Pretreatment Processes

for Lignocellulosic Biomass”, Wageningen UR

Food & Biobased Research: Wageningen.

Qureshi, N., B.C. Saha, M.A. Cotta, 2007.

“Butanol Production from Wheat Straw

Hydrolysate using Clostridium beijerinckii”.

Bioprocess Biosyst Eng. 30: 419–427.

Ishizawa, C.I., T. Jeoh, W.S. Adney, M.E.

Himmel, D.K. Johnson, M.F. Davis, 2009.

“Can delignification decrease cellulose

digestibility in acid pretreated corn stover?”

Cellulose, 16:677–686.

Lopéz-Contreras, 2000. “Utilisation of

Saccharides in Extruded Domestic Organic

Waste by Clostridium acetobutylicum ATCC

for Production of Acetone, Butanol

and Ethanol”. Applied Microbiology and

Biotechnology, Vol. 54, No. 2: 162-167.

Mosier, N., C. Wyman, B. Dale, R. Elander,

Y. Y. Lee, M. Holtzapple, and M. R. Ladisch,

“Features of Promising Technologies for

Pretreatment of Lignocellulosic Biomass”, Bioresource

Technology 96(6): 673-686.

Harun, R. and M.K. Danquah. 2011. “Infl uence

of Acid Pre-treatment on Microalgal Biomass for

Bioethanol Production”. Process Biochemistry,

: 304–309.

Qureshi, N., T.C. Ezeji, J. Ebener, B.S. Dien,

M.A. Cotta, H.P. Blaschek, 2008. “Butanol

Production by Clostridium beijerinckii. Part

I: Use of Acid and Enzyme Hydrolyzed Corn

Fiber”. Bioresource Technology, 99: 5915–

Rollin, J.A., Z. Zhu, N. Sathitsuksanoh, Y.H.

Percival Zhang, 2011. “Increasing Cellulose

Accessibility Is More Important Than

Removing Lignin: A Comparison of Cellulose

Solvent-Based Lignocellulose Fractionation

and Soaking in Aqueous Ammonia”.

Biotechnology and Bioengineering, 108 (1):

- 30.

Sánchez, O.J., C.A. Cardona (2008). “Trends in

biotechnological production of fuel ethanol from

dif-ferent feedstocks”, Bioresource Technololgy

: 5270-5295.

Selig, M., N. Weiss, and Y. Ji. 2008. “Enzymatic

Saccharifi cation of Lignocellulosic Biomass”,

Laboratory Analytical Procedure (LAP), Issue

Date: 3/21/2008, NREL, Colorado.

Saha, B.C., L.B. Iten, M.A. Cotta, Y. V. Wu,

“Dilute Acid Pretreatment, Enzymatic

Saccharifi cation, and Fermentation of Rice Hulls

to Ethanol”. Biotechnol. Prog. 21: 816-822.

Shang-Tian, Y. 2007. Bioprocessingfrom

Biotechnology to Biorefinery. in:

Bioprocessing for Value-added Products from

Renewable Resources: New Technology and

Apllications. Y. Shang-Tian (ed.). Elsevier,

Amsterdam.

Tashiro, Y. and K. Sonomoto. 2010. “Advances

in Butanol Production by Clostridia”, in: Current

Research and Education Topics in Applied

Microbiology and Microbial Biotechnology, A.

Mendez-Vilaz, (ed.), Formatex, Vol 2. p: 1383-




DOI: https://doi.org/10.29017/SCOG.35.1.776

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.