THE EXTRACTION OF OIL FROM Nannochloropsis sp. MICROALGAE USING ULTRASONIC AND FERMENTATION AS CELL DISRUPTION

Abstract

Algae are a promising source of biofuel but claims about their lipid content can be ambiguous because
extraction methods vary and lipid quantitation often does not distinguish between particular lipid classes.
One of algae types that meet this condition is Nannochloropsis sp. Two different cell disruption methods,i.e
ultrasonic and fermentation followed by maceration extraction using a mixture of ethanol/hexane (1:1, v/v)
as a solvent extraction were studied for their effectiveness in extraction of algae lipids from Nannochloropsis
sp. Contact time and amplitude were varied in ultrasonic process. Hydrolysis treatment was varied in
fermentation process, i.e Dillute Acid Pretreatment (DAP) hydrolysis, enzymatic hydrolysis and DAP with
enzymatic hydrolysis. The result showed that ultrasonic process followed by maceration extraction was
more effective for Nannochloropsis sp. lipid extraction. The most ideal treatment was at amplitude 40%
and contact time 5 minutes with lipid content of 63.59% of dry weight.