THE INFLUENCE OF MEDIA COMPOSITION TO THE GROWTH OF SCENEDESMUS SP. MICROALGAE IN VARIED MEDIA
Abstract
Media culture is one of the important factors in the growth of microalgae. Culture medium contains macro and micro nutrition required for the growth of microalgae. Composition of nutrition and nutrition concentrations determine the production of microalgae biomass and nutrient content. The aim of this study is to evaluate the effect of medium composition variation to Scenedesmus sp growth in laboratory-scale in order to get the optimized composition of media for microalgae Scenedesmus sp. This study was conducted in a laboratory scale. The growth medium that were used in this study were: BBL sm, SS, MN, M4N, “Sederhana 2”, and “Sederhana 3” medium. The microalgae that were used in this study were mixed culture Chlorophyceae microalgae which is dominated by Scenedesmus sp. The parameters observed were pH, optical density (OD), cell density and temperature. The results show that of the 6 variations of the growth medium used in this study M4N medium is the best medium for the growth of microalgae. At the end of the operation, cell density of Scenedesmus sp. from M4N medium was 306.83 x 106 cell/mL with a dark green color for the culture. The coefficient growth of Scenedesmus sp. which is grown in M4N medium was 0,36. This is likely to be due to the composition of nutrients in the medium M4N which were more complete than other media.
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Chang Ed-Haun dan Yang Shang-Shyng, 2003, “Some characteristics of microalgae isolated in Taiwan for biofixation of carbon dioxide”, Botanical Bulletin of Academia Sinica 44, p.43-52.
Chisti, Y., 2007, “Biodiesel from microalgae”, Biotechnology Advances 25:294-306.
Deng, X., Li, Y., & Fei, X., 2009, “Microalgae: Promising Feedstock for Biodiesel”, African Journal of Microbiology Research, Vol 3(13):1008-1014.
Dori, I., 2011, “Pemanfaatan Mikroalga Laut Scenedesmus Sp. Sebagai Penyerap Bahan Kimia Berbahaya Dalam air Limbah Industri”, Departemen Ilmu Dan Teknologi Kelautan Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor.
Fachrullah, R., 2011, Laju Pertumbuhan Mikroalga Penghasil Biofuel Jenis Chlorella sp dan Nannochloropsis sp yang Dikultivasi Menggunakan Air Limbah Hasil Penambangan Timah Di Pulau Bangka. Bogor: Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Kawaroe, M., Prartono, T., Sunuddin, A., Wulan, S. D., & Agustine, D., 2010, “Mikroalga Potensi dan Pemanfaatannya untuk Produksi Bio Bahan Bakar”, IPB Press, Bogor.
Graham, L.E. dan Wilcox, L.W., 2000, Algae, Prentice Hall, Inc., New Jersey.
Harrison, P.J and Berges, J.A., 2005, Marine culture media. In : Andersen, R.A (ed) Algal Culturing Techniques. Phycological Society of America, Elsevier, Academic Press, Amsterdam, pp 21-34.
Hu, H., & Gao, K., 2006. Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration. Biotechnol. Lett, 28, 987–992.
Mandal, Shovon., & Mallick, Nirupama., 2009, Microalga Scenedesmus obliquus as a potential source for biodiesel production, Appl Microbiol Biotechnol, 8: pp 281-291
Mata, T.M., Martins, A.A., & Caetano, S.N., 2010, “Microalgae for biodiesel production and other applications: A review”, Renewable and Sustainable Energy Reviews 14, pp 217–232.
Prihantini, B.N., Damayanti, D., & Yuniati, R., 2007, “Pengaruh Konsentrasi Medium Ekstrak Tauge (MET) Terhadap Pertumbuhan Scenedesmus Isolat Subang”, Makara Sains, Vol 11, No.1: pp 1-9.
Valderrama, Luz T., Del Campo, Claudia M., Rodriguez, Claudia M., de- Bashan, Luz E., and Bashan, Yoav, 2002, Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemna minuscula, water research, vol 36, pp 4185-4192.
DOI: https://doi.org/10.29017/SCOG.38.3.550
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