BUFFER REPLACEMENT AND SIMULTANEOUS SACCHARIFICATION AND FERMENTATION ON BIOBUTANOL PRODUCTION?FROM LIGNOCELLULOSIC BIOMASS

Devitra Saka Rani, Yanni Kussuryani

Abstract


Lignocellulosic biomass is excellent feedstock for biofuel such as biobutanol. Bagasse, rice straw, and empty fruit bunch (EFB) oil palm are untapped potential for biobutanol production as gasoline blending/ substitution. However, biobutanol production by fermentation from lignocellulosic biomass is a process that consumes time and energy which leads to high production costs. This research is intended to optimize biobutanol production that reduces production costs, an important factor on an industrial scale. Optimization is conducted by replacing the buffer solution in enzymatic hydrolysis with distilled water and by using Simultaneous Saccharification and Fermentation (SSF). The results showed that the buffer replacement with distilled water can reduce cost by approximately 41,726 IDR/liter hydrolysate. Biobutanol contents from all biomass of bagasse, rice straw, and EFB oil palm are higher using SSF compared to Separate Hydrolysis and Fermentation (SHF). The SSF system can cut production time by 3 days and save electricity of 32.4 kWh.?


Keywords


biofuel; biobutanol; lignocellulosic biomass; optimization; Simultaneous Saccharification and Fermentation

References


Aleksic, S., 2009, Butanol Production from Biomass, Thesis, Chemical Engineering, Youngstown State University, Youngstown, US

Arantes, V. & Saddler, J.N., 2010, Review: Access to Cellulose Limits the Efficiency of Enzymatic hydrolysis: The Role of Amorphogenesis, Biotechnology for Biofuels, 3:4

Banerjee, G., Car, S., Scott-Craig, J. S., Hodge1, D. B. & Walton, J. D., 2011, Alkaline Peroxide Pretreatment of Corn Stover: Effects of Biomass, Peroxide, and Enzyme Loading and Composition on Yields of Glucose and Xylose, Biotechnology for Biofuels, 4:16

Bisswanger, H., 2014, Review: Enzyme Assays, Perspectives in Science, 1 (1): 41-55

Carvalho, R. N. L., 2009, Dilute Acid and Enzymatic Hydrolysis of Sugarcane Bagasse for Biogas Production, Dissertation, Instituto Superior Tecnico, Lisboa

Dubey, R. C., 2014, Advanced Biotechnology. S Chand & Co Ltd, New Delhi, ISBN-10: 812194290X, ISBN- 13: 978-8121942904, pp: 265

Ezeji, T.C., Qureshi, N. & Blaschek, H.P., 2007, Bioproduction of Butanol from Biomass: from Genes to Bioreactors, Current Opinion in Biotechnology, 18:220227

Garca, V., Pkkil, J., Ojamo, H., Muurinen, E. & Keiski, R.L, 2011, Challenges in Biobutanol Production: How to Improve the Efficiency?, Renewable and Sustainable Energy Reviews, 15: 964980

Gupta, M. N., Mukherjee, J. & Malhotra, D., 2013, Use of High Activity Enzyme Preparations in Neat Organic Solvents for Organic Synthesis, Universal Organic Chemistry, Herbert Open Access Journal, ISSN 2053-7670, 1-11

Hambali, E., Thahar, A., Nisyaw, F. N., Biladi, D. B. C. & Haryanto, D., 2015, Peta Jalan Litbang Bahan Bakar Nabati Menuju Mandiri Energi (Road Map of Biofuel Research & Development Toward an Energy Independent), IPB Press, Bandung, ISBN: 979-979- 493-881-2, p 21-41

Harmsen, P., Huijgen, W., Bermudez, L. & Bakker, R., 2010, Literature Review of Physical and Chemical Pretreatment Processes for Lignocellulosic Biomass, Wageningen UR Food & Biobased Research, Wageningen

Kussuryani, Y. & Rani, D.S., 2015, Produksi Biobutanol sebagai Bahan Bakar Terbarukan melalui Proses Fermentasi (Biobutanol Production as a Renewable Fuel by Fermentation Process), Lembaran Publikasi Minyak dan Gas Bumi, 49(2): 101-110

Lee, S.Y., Park, J.H., Jang, S.H., Nielsen, L.K., Kim, J. & Jung, K.S., 2008, Fermentative Butanol Production by Clostridia, J.Biotechnology and Bioengineering, 101(2):209-228

Lpez, Y., Karimi, K., Taherzadeh, M.J. & Martin, C., 2011, Processing of Artisan Rice Hulls by Combinning Dilute-Acid Hydrolysis, Alkaline Delignification, NMMO Treatment and Enzymatic Hydrolysis, Science & Technology of Biomasses: Advences and Challenges, 5-8: 373-376.

Mosier, N., Wyman, C., Dale B., Elander, R., Lee, Y.Y., Holtzapple, M. & Ladisch, M. R., 2005, Features of Promising Technologies for Pretreatment of Lignocellulosic Biomass, Bioresource Technology, 96(6): 673-686

Promega, 2012, Protocol & Application Guide, Chapter 15: Buffer for Biochemical Reactions, rev.12/12, accessed on 25th April 2016 from www.promega.com

Qureshi, N., Ezeji, T.C., Ebener, J., Dien, B.S., Cotta, M.A. & Blaschek, H.P., 2008, Butanol Production by Clostridium beijerinckii. Part I: Use of Acid and Enzyme Hydrolyzed Corn Fiber, Bioresource Technology, 99: 59155922

Rani, D.S. & Sari, C.N., 2012, Dilute Acid Pretreatment and Enzymatic Hydrolysis of Lignocellulosic Biomass for Butanol Production as Biofuel, Scientific Contributions Oil and Gas, 35(1): 39-48

Thirmal, C., & Dahman, Y., 2011. Different Physi- cal and Chemical Pretreatments of Wheat Straw for Enhanced Biobutanol Production in Simultaneous Saccharification and Fermentation, International Journal of Energy And Environment, 2(4): 615-626

Xu, Jian, Thomsen, M. H. & Thomsen, A. B., 2009, Pretreatment on Corn Stover with Low Concentra-




DOI: https://doi.org/10.29017/SCOG.50.3.99

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.